polyclonal goat anti human endoglin antibody  (R&D Systems)

Journal: Frontiers in Medicine

Article Title: Endoglin and squamous cell carcinomas

doi: 10.3389/fmed.2023.1112573

Figure Lengend Snippet: Six marker FFPE panel for imaging mass spectrometry (Hyperion).

Article Snippet: Antigen retrieval was performed by boiling the sections in a 0.01 M citrate solution (pH 6.0) for 10 min. After being washed with phosphate-buffered saline (PBS), the sections were incubated with polyclonal goat anti-human endoglin (1:400—BAF1097, R&D systems, MN, United States) in PBS/1% bovine serum albumin (BSA), and left overnight at room temperature.

Techniques: Marker, Imaging, Mass Spectrometry

Journal: Frontiers in Medicine

Article Title: Endoglin and squamous cell carcinomas

doi: 10.3389/fmed.2023.1112573

Figure Lengend Snippet: Analysis of squamous cell carcinoma (SCC) primary tumors via immunohistochemistry, all tissues were stained for endoglin expression (brown). The black arrows indicate endothelial endoglin expression. The white arrows indicate epithelial endoglin expression. (A) Representative images of ESCC ( n = 9). (B) Representative images of HNSCC ( n = 5). (C) Representative images of VSCC ( n = 7). Images taken at 100x (left) and 200x (right).

Article Snippet: Antigen retrieval was performed by boiling the sections in a 0.01 M citrate solution (pH 6.0) for 10 min. After being washed with phosphate-buffered saline (PBS), the sections were incubated with polyclonal goat anti-human endoglin (1:400—BAF1097, R&D systems, MN, United States) in PBS/1% bovine serum albumin (BSA), and left overnight at room temperature.

Techniques: Immunohistochemistry, Staining, Expressing

Journal: Frontiers in Medicine

Article Title: Endoglin and squamous cell carcinomas

doi: 10.3389/fmed.2023.1112573

Figure Lengend Snippet: Analysis of HNSCC via imaging mass spectrometry (Hyperion) with a six marker panels. (A) Six images, each depicting the expression of the corresponding marker. (B) A merged image combining pan-cytokeratin, endoglin, and p53 expression. The white arrow indicates cells that co-express pan-cytokeratin and endoglin, which are negative for p53. (C) A merged image combining pan-cytokeratin, endoglin, and CD68 expression. The white arrow indicates cells that co-express pan-cytokeratin and endoglin, which are negative for CD68.

Article Snippet: Antigen retrieval was performed by boiling the sections in a 0.01 M citrate solution (pH 6.0) for 10 min. After being washed with phosphate-buffered saline (PBS), the sections were incubated with polyclonal goat anti-human endoglin (1:400—BAF1097, R&D systems, MN, United States) in PBS/1% bovine serum albumin (BSA), and left overnight at room temperature.

Techniques: Imaging, Mass Spectrometry, Marker, Expressing

Journal: Frontiers in Medicine

Article Title: Endoglin and squamous cell carcinomas

doi: 10.3389/fmed.2023.1112573

Figure Lengend Snippet: Analysis of ESCC via imaging mass spectrometry (Hyperion) with a six marker panels. (A) Six images, each depicting the expression of the corresponding marker. (B) A merged image combining pan-cytokeratin, endoglin, and p53 expression. The white arrows indicate cells that co-express pan-cytokeratin and endoglin, which are negative for p53. (C) A merged image combining pan-cytokeratin, endoglin, and CD68 expression. The white arrow indicates cells that co-express pan-cytokeratin and endoglin, which are negative for CD68. The blue arrow indicates cells that co-express pan-cytokeratin, endoglin, and CD68.

Article Snippet: Antigen retrieval was performed by boiling the sections in a 0.01 M citrate solution (pH 6.0) for 10 min. After being washed with phosphate-buffered saline (PBS), the sections were incubated with polyclonal goat anti-human endoglin (1:400—BAF1097, R&D systems, MN, United States) in PBS/1% bovine serum albumin (BSA), and left overnight at room temperature.

Techniques: Imaging, Mass Spectrometry, Marker, Expressing

Journal: Frontiers in Medicine

Article Title: Endoglin and squamous cell carcinomas

doi: 10.3389/fmed.2023.1112573

Figure Lengend Snippet: Analysis of VSCC via imaging mass spectrometry (Hyperion) with a six marker panel. (A) Six images, each depicting the expression of the corresponding marker. (B) A merged image combining pan-cytokeratin, endoglin, and p53 expression. The white arrows indicate cells that co-express pan-cytokeratin and endoglin. (C) A merged image combining pan-cytokeratin, endoglin, and CD68 expression. The white arrow indicates cells that co-express pan-cytokeratin and endoglin, which are negative for CD68. The blue arrow indicates cells that co-express pan-cytokeratin, endoglin, and CD68.

Article Snippet: Antigen retrieval was performed by boiling the sections in a 0.01 M citrate solution (pH 6.0) for 10 min. After being washed with phosphate-buffered saline (PBS), the sections were incubated with polyclonal goat anti-human endoglin (1:400—BAF1097, R&D systems, MN, United States) in PBS/1% bovine serum albumin (BSA), and left overnight at room temperature.

Techniques: Imaging, Mass Spectrometry, Marker, Expressing

Journal: Frontiers in Medicine

Article Title: Endoglin and squamous cell carcinomas

doi: 10.3389/fmed.2023.1112573

Figure Lengend Snippet: The expression of endoglin by SCC cell lines. Expression by 10 ESCC cell lines, where endoglin expression by TE01 ( p < 0.0001) and TE15 ( p < 0.003) significantly differ from all other cell lines (A) , OSC-19 and FaDu show significantly different endoglin expression ( p = 0.0002) (B) , as is also detected in the three VSCC cell lines (* p = 0.0123, ** p = 0.0025, *** p = 0.0001) with different morphologies (conventional—VC415-C and VC704; spindle—VC415-S) (C) . Endoglin protein levels were determined via western blot (D) and ELISA. Endoglin protein expression by TE01 significantly differs from all other cell lines—( p ≤ 0.0018) (E) . Image for western blot analysis is a representative image of n = 2–3 independent experiments.

Article Snippet: Antigen retrieval was performed by boiling the sections in a 0.01 M citrate solution (pH 6.0) for 10 min. After being washed with phosphate-buffered saline (PBS), the sections were incubated with polyclonal goat anti-human endoglin (1:400—BAF1097, R&D systems, MN, United States) in PBS/1% bovine serum albumin (BSA), and left overnight at room temperature.

Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Medicine

Article Title: Endoglin and squamous cell carcinomas

doi: 10.3389/fmed.2023.1112573

Figure Lengend Snippet: Analysis of ALK expression by SCC cell lines, determined via qPCR. Gene expression for ALK1—ALK7 by TE10—endoglin low (A) , TE11—endoglin low (B) , TE01—endoglin high (C) , OSC-19—endoglin positive (D) , FaDu–endoglin high (E) , VC415-C—endoglin low, and VC415-S—endoglin high; *** p = 0.000581 (F) . The effect of endoglin overexpression (OE) on ALK expression was analyzed for TE10; *** p =0.000126; ** p =0.003291 (G) and TE11 (H) , as well as the effect of endoglin knockout (KO) in TE01 (I) .

Article Snippet: Antigen retrieval was performed by boiling the sections in a 0.01 M citrate solution (pH 6.0) for 10 min. After being washed with phosphate-buffered saline (PBS), the sections were incubated with polyclonal goat anti-human endoglin (1:400—BAF1097, R&D systems, MN, United States) in PBS/1% bovine serum albumin (BSA), and left overnight at room temperature.

Techniques: Expressing, Gene Expression, Over Expression, Knock-Out

Journal: Frontiers in Medicine

Article Title: Endoglin and squamous cell carcinomas

doi: 10.3389/fmed.2023.1112573

Figure Lengend Snippet: SCC cells were stimulated with either BMP-6 (TE10 and TE11 only), BMP-9 or TGF-β and the level of phosphorylated SMAD1 and SMAD2 (pSMAD1 and pSMAD2) was determined via western blot (A–C) . The amount of soluble endoglin in the medium of TE10 and TE11 was determined via ELISA (D,E) . Stimulation of OSC-19 (F) , FaDu (G) , VC415-C (H) , and VC415-S (I) with BMP-9/TGF-β/TRC105 was performed, and the levels of pSMAD1 and pSMAD2 were determined via western blot. Western blot images are representative of n = 2–3 per experiment.

Article Snippet: Antigen retrieval was performed by boiling the sections in a 0.01 M citrate solution (pH 6.0) for 10 min. After being washed with phosphate-buffered saline (PBS), the sections were incubated with polyclonal goat anti-human endoglin (1:400—BAF1097, R&D systems, MN, United States) in PBS/1% bovine serum albumin (BSA), and left overnight at room temperature.

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Medicine

Article Title: Endoglin and squamous cell carcinomas

doi: 10.3389/fmed.2023.1112573

Figure Lengend Snippet: SCC cell lines were stimulated with BMP-9 or TGF-β and the proliferation of TE10 (A) , TE11 (C) , TE01 (E) , and VC415-S (G) cells was measured via a MTS assay. To assess the effects of endoglin on cell proliferation, MTS assays were performed on endoglin overexpressing (OE) TE10 (B) and TE11 (D) cells. The effects of endoglin knockout (KO) in TE01 (F) and endoglin knockdown (KD) in VC415-S (H) were also assessed via MTS. n = 2–3 for each experiment.

Article Snippet: Antigen retrieval was performed by boiling the sections in a 0.01 M citrate solution (pH 6.0) for 10 min. After being washed with phosphate-buffered saline (PBS), the sections were incubated with polyclonal goat anti-human endoglin (1:400—BAF1097, R&D systems, MN, United States) in PBS/1% bovine serum albumin (BSA), and left overnight at room temperature.

Techniques: MTS Assay, Knock-Out, Knockdown

Journal: Frontiers in Medicine

Article Title: Endoglin and squamous cell carcinomas

doi: 10.3389/fmed.2023.1112573

Figure Lengend Snippet: SCC cell lines were stimulated with BMP-9 or TGF-β and the migration of TE10 (A) , TE11 (C) , TE01 (E) , VC415-S (G) , OSC-19 (I) , and FaDu (J) cells was measured via a wound healing assay. To assess the effects of endoglin on cell migration, wound healing assays were performed on endoglin overexpressing (OE) TE10 (B) and TE11 (D) cells. The effects of endoglin knockout (KO) in TE01 (F) and endoglin knockdown (KD) in VC415-S (H) were also assessed. Finally, the effects of TRC105 on cell migration was assessed in OSC-19 (I) and FaDu cells (J) . n = 2–3 for each experiment.

Article Snippet: Antigen retrieval was performed by boiling the sections in a 0.01 M citrate solution (pH 6.0) for 10 min. After being washed with phosphate-buffered saline (PBS), the sections were incubated with polyclonal goat anti-human endoglin (1:400—BAF1097, R&D systems, MN, United States) in PBS/1% bovine serum albumin (BSA), and left overnight at room temperature.

Techniques: Migration, Wound Healing Assay, Knock-Out, Knockdown

Journal: Frontiers in Medicine

Article Title: Endoglin and squamous cell carcinomas

doi: 10.3389/fmed.2023.1112573

Figure Lengend Snippet: Summary table of the relationship between (altered) endoglin expression, BMP-9 signaling, TGF-β signaling, SCC cell migration, and SCC cell proliferation.

Article Snippet: Antigen retrieval was performed by boiling the sections in a 0.01 M citrate solution (pH 6.0) for 10 min. After being washed with phosphate-buffered saline (PBS), the sections were incubated with polyclonal goat anti-human endoglin (1:400—BAF1097, R&D systems, MN, United States) in PBS/1% bovine serum albumin (BSA), and left overnight at room temperature.

Techniques: Expressing, Migration

Journal: Biomaterials

Article Title: Tumor invasion unit in gastric cancer revealed by QDs-based in situ molecular imaging and multispectral analysis.

doi: 10.1016/j.biomaterials.2014.01.059

Figure Lengend Snippet: Fig. 2. The QDs-based in situ molecular imaging and multispectral analyses of tumor invasion unit in GC. (A) Tumor slides from GC specimens were first stained with 2 primary an- tibodies against macrophages and CD105, a marker of tumor neo-vessels; and then stained with QDs secondary antibodies, with the macrophages stained green (525 nm spectrum) and neo-vessels stained red (655 nm spectrum). (B, C) The images were computer captured and unmixed by multispectral analysis software, to delete the signal noise and set the spectral images of macrophages and neo-vessels for subsequent analysis. (D) With green signal of a macrophage as the center, a 60 mm-diameter circle was set to see if there were any red signals of neo-vessels in this circle. Only circles with both macrophage and neo-vessel signals were considered as tumor invasion units. The total number of circles was counted as the final output results of tumor invasion unit. GC: gastric cancer. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: For QDs-based double molecular imaging, the primary antibodies were mouse anti-human monoclonal antibody against macrophages (MA1-38069, ABR, USA; dilution 1/300) and goat anti-human polyclonal antibody against CD105 (sc-20072, Santa Cruz, USA; dilution 1/300).

Techniques: In Situ, Imaging, Staining, Marker, Software